Freshly isolated hepatocytes are widely accepted as a superior in-vitro model for evaluating the in-vitro hepatic metabolism and toxicity of test compounds because they more closely reflect the in-vivo situation, when compared with other models.
Biopredic International has built up a well-organized supply network which guarantees the regular delivery of human liver resects (after partial hepatectomy or from human whole livers rejected for liver transplantation) and animal livers. High quality fresh human and animal hepatocytes are isolated and then fully characterized.
Fresh human hepatocytes products include
- Hepatocytes suspensions suitable for short-term metabolism studies (up to 4 hours) and
- Plated (monolayer) hepatocytes cultures for longer-term metabolism studies (up to 3 days).
Fresh human hepatocytes in suspension
Fresh plated human hepatocytes
Fresh Animal Hepatocytes
Biopredic International provides a wide range of fresh animal hepatocytes obtained from a variety of species: rat, mouse, hamster, rabbit, cynomolgus monkey, dog, minipig and other species (available on demand).
Fresh animal hepatocytes can be prepared from either single animal liver donor (such as mouse, rat, dog, monkey, rabbit or hamster) or pooled from several animal donors (mouse, rat or rabbit) according to our production schedule.
All rodent hepatocytes are isolated from animals housed in our animal facility which is managed in strict compliance with the Directive 2010/63/EU of the European Parliament and of the Council.
Hepatocytes of other animal species are isolated from the liver tissues collected following approved protocols and respecting all ethical and legal requirements.
Fresh animal hepatocytes products include
- Hepatocytes suspensions
- Plated (monolayer) hepatocytes cultures
Fresh Animal Hepatocytes in suspension
Fresh Plated Animal Hepatocytes
Biopredic International has more than 20 years in-house experience of cryopreserving human and animal hepatocytes.
Biopredic offers hepatocytes in suspension (Grade S) or plateable.
Plateable hepatocytes are offered in different grades:
- Cryoplateable Human Hepatocytes (Grade P)
- Cryoplateable and inducible Human Hepatocytes (Grade I)
- Cryoplateable & easy-to-use Hepatocytes (Grade NS for “no-spin required”)
- Cryoplateable & sandwich culture hepatocytes
Biopredic International is also the sole European distributor of cryopreserved hepatocytes produced by Triangle Research Labs (TRL), offering a wider supply of human hepatocytes.
Every lot of human cryopreserved hepatocytes from TRL is fully characterized for viability/stability, cell yield, attachment efficiency (and optimal seeding density), monolayer confluency, Phase I and II drug enzyme activities, induction potential, transporter activity, and 14 relevant SNPs.